The efficacy of Ready-to-Use Supplementary Foods (RUSF) depends critically on adequate micronutrient bioavailability and consumer acceptability, presenting particular challenges for plant-based formulations. This study comprehensively evaluated the micronutrient composition of a novel plant-based RUSF cookie formulated from indigenous Nigerian ingredients. Two cookie formulations (FPP A for MAM/convalescence; FPP B for general health) were analyzed for vitamin content using HPLC, spectrophotometric methods, and mineral content using Atomic Absorption Spectrophotometry. FPP A demonstrated exceptional mineral content in phosphorus (752.66 ± 1.11 mg/100kcal), potassium (1320.79 ± 0.18 mg/100kcal), and magnesium (213.71 ± 0.68 mg/100kcal), significantly exceeding RUSF standards (p<0.05). However, iron (11.38 ± 0.09 mg/100kcal) and zinc (10.09 ± 0.12 mg/100kcal) contents were slightly below standards. Vitamin analysis revealed adequate levels of thiamine (1.45 ± 0.02 mg/100g), pyridoxine (2.53 ± 0.02 mg/100g), folate (343.41 ± 1.22 mg/100g), vitamins D and E, but significant deficiencies in vitamins A (343.81 ± 0.48 μg/100g), B2 (1.52 ± 0.02 mg/100g), B3 (10.57 ± 0.08 mg/100g), and C (57.83 ± 0.22 mg/100g). The plant-based RUSF cookie demonstrates excellent mineral bioavailability potential and good B-vitamin content but requires strategic fortification to address iron, zinc, and specific vitamin deficiencies.