Background: Gender dysphoria refers to the distress accompany the incongruence between one's experienced or expressed gender and the feeling of discomfort or distress that might occur in people whose gender identity differs from their sex assigned at birth or sex-related physical characteristics; it is a revision of gender identity disorder. This revision was made in support of tarn’s individuals seeking treatment or care options such as counselling, hormone treatments, gender confirmation surgery, and/or a legal name and gender change. The aim of this study was to assess nurse’s knowledge and attitude regarding gender dysphoria patients. Subjects and Methods: Descriptive cross – sectional hospital base study design, was carried out, sample size taken was 100 nurses, the sampling technique was total coverage to all nurses working at Ibn Sina hospital, Inclusion criteria, nurses working at Ibn Sina hospital their age (20 -60) year old Data was collected by structured Self- administered questionnaire, which consist of 3 parts, sociodemographic data, nurses knowledge about gender dysphoria, and their attitude towards gender dysphoria patients, Data analysis done using statistical package for social science version 20, descriptive statistic as well as chi-square test were done for association. Results: Demographic Characteristics 65% of participants aged 20–29, 17% 30–39, 18% 40–49 consecutively. females were 91% and 9% were male. Educational qualifications; 80% held a Bachelor's degree, 19% held a Master's degree and 1% held a PhD. Years of Experience; 68% had 1–5 years of experience, 8% had 6–10 years, and 24% had 11–15 years. Knowledge regarding gender dysphoria general understanding; 86% recognized gender dysphoria as an asexual behaviour disorder, 93% understood it as persistent distress with one’s gender identity, 74% knew it involves identifying with the opposite sex. Treatment knowledge;83% believed gender dysphoria can be treated with therapy, 72% supported the use of hormone therapy, 89% recognized patients might exhibit opposite sex behaviour, 83% believed males may dress/behave like women, 82% believed females may dress/behave like men, 87% stated patients may try to hide secondary sexual characteristics. Nurses’ attitudes toward gender dysphoria Patients; 74% strongly agreed that nurses must accept the patient, reduce anxiety, and provide appropriate care. Community participation and family Involvement; 74% strongly agreed that nurses must involve families and develop community participation. Conclusion and Recommendations: The majority of nurses demonstrated a good level of knowledge regarding gender dysphoria, including its causes, symptoms, and treatment options. Most participants also showed a positive and professional attitude toward patients, emphasizing acceptance, non-judgment, and inclusive care practices. However, a minority of responses indicate a need for further education to ensure consistent and comprehensive understanding among Nurses.